TABLE 2.
Injected material and expt no | No. of embryos
|
% of embryos with abnormal phenotype
|
|||
---|---|---|---|---|---|
Injected | With fluorescence | With abnormal phenotype | In each expt | Avg ± SD for group | |
pcXGFP | 0 | ||||
1 | 199 | 121 | 0 | 0 | |
2 | 189 | 107 | 0 | 0 | |
3 | 167 | 98 | 0 | 0 | |
pG48R | 53.58 ± 0.89 | ||||
1 | 59 | 32 | 32 | 54.2 | |
2 | 89 | 48 | 48 | 53.9 | |
3 | 78 | 41 | 41 | 52.6 | |
pGV121 | 45.53 ± 1.93 | ||||
1 | 194 | 84 | 84 | 43.3 | |
2 | 146 | 68 | 68 | 46.6 | |
3 | 152 | 71 | 71 | 46.7 | |
pG48R plus pGV121 | 74.70 ± 1.82 | ||||
1 | 98 | 72 | 72 | 73.5 | |
2 | 112 | 86 | 86 | 76.8 | |
3 | 107 | 79 | 79 | 73.8 |
The embryos were at the stage of 48 hpf. The data shown here were collected from three individual injection experiments. The embryos were injected at the one-cell stage. Approximately 0.15 to 0.2 ng of pcXGFP, pG48R, pGV121, and pG48R plus pGV121 was injected in each embryo.