FIG. 4.

Capsid expression is associated with redistribution of PABP. Vero cells were transfected with plasmids encoding capsid or glycoproteins E2 and E1. Forty hours posttransfection, cells were processed for indirect immunofluorescence using rabbit anticapsid (α-capsid) or human anti-RV serum (α-PABP) to detect E1 and E2. PABP was detected using a mouse monoclonal antibody. Nuclei were stained with DAPI. The arrowheads indicate areas with extensive colocalization between PABP and capsid. Note because the PABP signal was so much higher in cells expressing capsid, to avoid saturation in the red channel, it was necessary to use shorter exposure times for the images shown in the top row. As a result, the PABP signal in the untransfected cells appears lower than those in the bottom two rows. Bar, 10 μm.