FIG. 2.

Analysis of induction of UPRE and ERSE promoters during MHV infection. DBT cells were transfected with either UPR reporter plasmid pUPRE-GL3 or ERSE reporter plasmid pERSE-GL3 DNA and, 24 h later, infected with MHV or treated with tunicamycin (Tun; 2 μg/ml). Cell lysates were prepared at the times indicated and assayed in triplicate using a dual-luciferase assay as described in Materials and Methods. Error bars show standard deviations.