FIG. 5.

Influence of p6* mutations on viral in vitro replication and infectivity. (A) MT-4 lymphocytes were transiently transfected with the indicated provirus clones. Cells were subcultivated at a 1:2 ratio every 48 h, and the total amount of virus-associated CA antigen in culture supernatants was determined by an ELISA. The data shown are mean values from two independent transfections performed in duplicate experiments with different DNA preparations, and the error bars indicate the standard deviations. (B) Magi indicator cells were infected with different dilutions of wt and mutant viruses obtained from transient transfections of H1299 cells and normalized to the amount of CA amount as described in Materials and Methods. Forty-eight hours postinfection, Magi cells were fixed and stained, and blue nuclei in each well were counted. The data shown are the mean values from three independent infections, and the standard deviations (error bars) are indicated.