TABLE 1.
Primers used for generation of p6* mutants, reporter constructs, and sequencing
| Primer | Primer sequence (5′→3′) | Nucleotide positions within HX10a |
|---|---|---|
| M1 | AGACAGGCTAATTTTTTTGGGAAGATCTGGCCTTCC | 1284-1319 |
| GGAAGGCCAGATCTTCCCAAAAAAATTAGCCTGTCT | 1319-1284 | |
| M2 | GCTAATTTTTTAGGGAAGATCTGGCCGTCCTACAAG | 1290-1325 |
| CTTGTAGGACGGCCAGATCTTCCCTAAAAAATTAGC | 1325-1290 | |
| M3 | CAAGGGACGGCCAGGGAATTTTCTTCAGAGC | 1322-1352 |
| GCTCTGAAGAAAATTCCCTGGCCGTCCCTTG | 1352-1322 | |
| M4a | GGAACTGTACCCTTTAACCTCCCTCAGATCACTCTTTGG | 1478-1516 |
| CCAAAGAGTGATCTGAGGGAGGTTAAAGGGTACAGTTCC | 1516-1478 | |
| M4b | GGAACTGTACCCTCTCACCTCCCTCAGATCACTCTTTGG | 1478-1516 |
| CCAAAGAGTGATCTGAGGGAGGTGAGAGGGTACAGTTCC | 1516-1478 | |
| M5 | GGAACTGTACCCATTGACCTCCCTCAGATCACTCTTTGG | 1478-1516 |
| CCAAAGAGTGATCTGAGGGAGGTCAATGGGTACAGTTCC | 1516-1478 | |
| M6 | GGAACTGTACCCTTTGACTTCCCTCAGATCACTCTTTGG | 1478-1516 |
| CCAAAGAGTGATCTGAGGGAAGTCAAAGGGTACAGTTCC | 1516-1478 | |
| fs1 | AGACAGGCTAATTTTTTTGGGAAGATCTGGCCTTCC | ND |
| GGAAGGCCAGATCTTCCCAAAAAAATTAGCCTGTCT | ||
| fs2 | GCTAATTTTTTAGGGAAGATCTGGCCGTCCTACAAG | ND |
| CTTGTAGGACGCCAGATCTTCCCTAAAAAATTAGC | ||
| fs3 | CCTACAAGGGACGGCCAGGGAAATCC | ND |
| GGATCCTTCCCTGGCCGTCCCTTGTAGG | ||
| S1f | GCACCAGGCCAGATGAGAGAACC | 669-690 |
| S2f | GCATTGGGACCAGCGGCTACAC | 1005-1025 |
| S3f | CTACAAGGGAAGGCCAGGG | 1319-1337 |
| S4r | GGATACAGTTCCTTGTCTATCGGC | 1489-1466 |
| S5r | CCATTGTTTAACTTTTGGGCC | 1866-1847 |
| S6f | GGAAATGTGGAAAGGAAGGACACC | 1240-1262 |
| S7r | CTGTCTTACTTTGATAAAACCTCC | 1671-1649 |
a
Nucleotide positions within HX10 (48) with respect to Gag ATG. ND, not determined.