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. 2008 Feb 27;82(9):4235–4249. doi: 10.1128/JVI.02370-07

FIG. 1.

FIG. 1.

Overexpression of the vFLIP gene in PEL cells inhibited KSHV lytic replication. BCBL-1 cells stably transduced with vFLIP (MSCV-vFLIP) or a vector control (MSCV) with or without induction with T/B were analyzed for KSHV lytic replication. (A) Western blot analysis of vFLIP and RTA proteins. Western blotting for β-tubulin was also carried out to calibrate protein loading. (B and C) Quantification of expression levels of vFLIP (B) and RTA (C) proteins. The relative intensities of the bands were calibrated with their corresponding β-tubulin bands. Uninduced MCSV cells were set as 1. (D and E) Analysis by RT-qPCR of vFLIP (D) and RTA (E) transcripts. RT-qPCR for GAPDH (glyceraldehyde-3-phosphate dehydrogenase) transcripts was also carried out for calibrating sample inputs. (F and G) Results of IFAs for ORF59 (F) and ORF65 (G) proteins. (H) Representative images showing that MSCV-vFLIP cells produced fewer KSHV virions than MSCV cells following induction with T/B. (I) Relative virus titers produced by MCSV and MSCV-vFLIP cells with or without induction with T/B. +, presence; −, absence.