TABLE 1.

PCR primers used, melting temperatures of the amplified products, and copy numbers detected

Gene	Primer and sequence	Tma (7deg;C)	Nb
ORF50	ORF50F: 5′-CACAAAAATGGCGCAAGATGA-3′	82	50
	ORF50R: 5′-TGGTAGAGTTGGGCCTTCAGTT-3′
ORF57	ORF57F: 5′-ACGAATCGAGGGACGACG-3′	82.5	50
	ORF57R: 5′-CGGGTTCGGACAATTGCT-3′
ORF59	ORF59F: 5′-CGAGTCTTCGCAAAAGGTTC-3′	80.2	50
	ORF59R: 5′-AAGGGACCAACTGGTGTGAG-3′
ORF65	ORF65F: 5′-ATATGTCGCAGGCCGAATAC-3′	77.4	5
	ORF65R: 5′-CCACCCATCCTCCTCAGATA-3′
ORF71	ORF71F: 5′-GGATGCCCTAATGTCAATGC-3′	82	5
	ORF71R: 5′-GGCGATAGTGTTGGGAGTGT-3′
ORF72	ORF72F: 5′-GCTGATAATAGAGGCGGGCAATGAG-3′	80	5
	ORF72R: 5′-GTTGGCGTGGCGAACAGAGGCAGTC-3′
ORF73	ORF73F: 5′-GCAGACACTGAAACGCTGAA-3′	81.5	50
	ORF73R: 5′-AGGTGAGCCACCAGGACTTA-3′
K8.1	K8.1F: 5′-AAAGCGTCCAGCCCACCACAGA-3′	80.2	5
	K8.1R: 5′-GGCAGAAAATGGCACACGGTTAC-3′
K8	K8F: 5′-CATGCTGATGCGAATGTGC-3′	82	50
	K8R: 5′-AGCTTCAACATGGTGGGAGTG-3′
GAPDH	GAPDH-F: 5′-CCCCTGGCCAAGGTCATCCA-3′	83	5
	GAPDH-R: 5′-ACAGCCTTGGCAGCGCCAGT-3′

^aMelting temperature of the amplified product.

^bLowest copy number that could be detected.