FIG. 1.

Ad carries an E3 gene that downregulates cell surface MICA/B. (A) HFFF infected at a multiplicity of infection of 50 with a replication-deficient (ΔE1,E3) HAdV-5 vector (RAd60) or wt HAdV-5 were stained at 48 h p.i. with an anti-MICA/B antibody or control IgG and analyzed by flow cytometry. The mouse IgG control is shown by the thin line, and the filled histograms represent staining with anti-MICA/B. The mean fluorescence intensity of MICA/B staining of mock-, RAd60-, or HAdV-5-infected cells is depicted in the top right corner of the histogram. (B) E3 genes present in each of the three viruses. (C) 911 cells infected at a multiplicity of infection of 5 with wt HAdV-5, RAd60, or RAd592 were stained at 24 h p.i. with anti-MICA/B antibody or control IgG before analysis by flow cytometry. The mouse IgG control is shown by the thin line, staining of mock-infected cells is in gray fill, and staining of infected cells is represented by the bold line. The mean fluorescence intensity of MICA/B staining of HAdV-5-, RAd60-, or RAd592-infected cells is depicted in the top right corner of the histogram.