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. 2008 Feb 20;82(9):4585–4594. doi: 10.1128/JVI.02251-07

FIG. 4.

FIG. 4.

E3/19K inhibits posttranslational maturation of MICA/B. (A) Lysates from the 293 cell lines indicated were either mock digested (M) or digested with EndoH (E) or PNGase F (P) before immunoblotting with a MICA/B-specific polyclonal antibody. Arrowheads denote the mature EndoH-resistant forms, and arrows denote the EndoH-sensitive forms. (B) Lysates generated from HF cells infected at a multiplicity of infection of 3 with the indicated viruses at 48 h p.i. were mock digested or digested with EndoH or PNGase F before immunoblotting with a MICA/B-specific polyclonal antibody. Arrowheads denote the EndoH-resistant forms. (C) Lysates as described for panel B were immunoblotted with anti-MHC-I monoclonal antibody (HC10). (D) Lysates as described for panel B were immunoblotted with an antiactin antibody as a loading control.