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. 2008 Feb 25;28(9):2952–2970. doi: 10.1128/MCB.00248-08

FIG. 1.

FIG. 1.

PKA inhibits myogenic differentiation. (A) C2C12 myoblasts were transiently transfected with pMCK-EGFP and pCMV-dsRed2, with or without the pFC-PKA (catalytic subunit of PKA) vector, recovered in GM for 24 h, and then induced to differentiate by using 5% horse serum (DM). After 48 or 72 h in DM, phase-contrast and fluorescence images (green, pMCK-EGFP; and red, pCMV-dsRed2) were obtained. MT, myotubes. (B) C2C12 cells were transiently transfected with pMCK-Luc and increasing amounts of pFC-PKA (100 to 500 ng) and harvested 48 h after being induced to differentiate in DM. Overexpression of PKA significantly suppressed MCK-Luc in a dose-dependent manner. Data are means ± SEM (n = 3). (C) C3H10T1/2 cells were cotransfected with empty vector or pcDNA3-MEF2D and increasing amounts of pFC-PKA (0.02 to 0.2 μg) and harvested 48 h after transfection. pGL3-4 × MEF2-Luc (four copies of MEF2 binding sites driven by the luciferase reporter gene) was used as the reporter gene.