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Akt activation by EGF is suppressed in RalGDS-depleted MCF-7 cells. (A) Expression levels of RalGDS detected by RT-PCR in MCF-7 cells transfected with RalGDS siRNA or control siRNA. (B) MCF-7 cells transfected with RalGDS siRNA or control siRNA were serum starved for 6 h and then stimulated with EGF (5 ng/ml) for 5 min, and cell lysates were assayed for pAkt308, pAkt473, total Akt, and pERK. Results are representative of experiments performed at least three times.
