FIG. 8.

Enhanced cell proliferation and elevated Akt activation both depend upon the N terminus of RalGDS. (A) Pools of NIH 3T3 cells stably expressing empty vector, Myc-RalGDS (GDS), or Myc-RalGDSΔN (ΔN) were lysed and assayed for pAkt308, pAkt473, total Akt, and Myc-GDS. (B) Pools of NIH 3T3 cells stably expressing empty vector, Myc-RalGDS, or Myc-RalGDSΔN were plated at 2 × 10⁴ cells/ml in a 12-well plate in low serum medium (0.25%). Cell numbers were determined at the indicated times. The data represent the averages of data from three independent experiments.