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. 2008 Feb 11;28(8):2758–2770. doi: 10.1128/MCB.01704-07

FIG. 1.

FIG. 1.

The 5.7-kb upstream region of the Keap1 gene (KRD) recapitulates the endogenous expression profile. (A) Schematic structures of the wild-type Keap1 gene, its targeted allele, and the KRD-LacZ transgene. The 5.7-kb genomic fragment of the Keap1 gene contains the upstream region with three alternative noncoding first exons and part of the second exon. Neo, neomycin resistance cassette. (B to S) LacZ expression observed in KRD-LacZ mice (D, G, J, M, P, and S), Keap1+/− mice (C, F, I, L, O, and R), and wild-type mice (B, E, H, K, N, and Q). The LacZ expression profiles observed for KRD-LacZ mice were compared to those for Keap1+/− mice, in which LacZ was expressed from the targeted allele under the regulation of the endogenous Keap1 gene. Skin (B to D), esophagus (E to G), and forestomach (H to J) tissue samples were examined at E18.5. Lung (K to M), liver (N to P), and heart (Q to S) tissue samples were examined at P1. Scale bars correspond to 400 μm (B to G), 200 μm (H to P), and 2 mm (Q to S).