FIG. 4.

(A) Alignment of Keap1 cysteine residues modified by electrophiles in vitro. Three kinds of electrophiles, dexamethasone 21-mesylate, biotinylated iodoacetamide, and sulforaphane, were applied, and the cysteine residues modified by each reagent are indicated. Physiologically relevant cysteine residues identified in the transgenic complementation rescue analysis in our present study are highlighted in blue or red. #a, #b, #c, #d, and #e indicate references 3, 4, 8, 9, and 5, respectively. (B) Schematic structures of the mutant Keap1 molecules examined in this study. By using these mutant Keap1 molecules, the evaluation of the Keap1 repressor function was executed as an index of the survival of Keap1 null mice carrying a mutant Keap1 transgene expressing a KRD under the regulation of DGR, double glycine repeat.