FIG. 8.
The Keap1 C151S mutant molecule rescues Keap1 null mice from lethality but compromises Nrf2 activation. (A) Quantitative expression analysis of the Keap1 C151S mutant transgene in the forestomachs of Keap1−/−::TgC151S mice. Values relative to the endogenous Keap1 levels in wild-type mice are presented. Triplicate samples were analyzed, and the means and standard errors are indicated. (B) Growth curves for independent lines of Keap1−/−::TgC151S mice. (C to E) Histological examination of forestomachs and esophagi of Keap1−/−::TgC151S mice. Forestomachs (C and D) and esophagi (E) of line 16 Keap1−/−::TgC151S mice at P10 were stained with hematoxylin and eosin. (C) Sagittal section of a whole stomach. White and black arrowheads indicate the cardiac part and the pyloric segment of the stomach, respectively. (D) High-power magnification of the limiting ridge region (the region within the square in panel C). The scale bar corresponds to 400 μm.