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. 2008 Feb 11;28(8):2579–2589. doi: 10.1128/MCB.01795-07

FIG. 3.

FIG. 3.

Phosphorylated Mpk1 and Mlp1 bind to the FKS2 promoter. (A) ChIP analysis of the FKS2 promoter with Mpk1-HA. A wild-type yeast strain (1788) was cotransformed with FKS2-lacZ reporter plasmids (p2052, wild-type; or p2053, SBF-site mutant −386T) and multicopy plasmids expressing the indicated HA-tagged MPK1 allele (wild-type, p777; mpk1-TA/YF, p778; mpk1-K54R, p2119; or vector, YEp351). Transformants were cultivated in YEPD medium at 23°C or subjected to thermal stress at 39°C for 15 h prior to ChIP analysis using primers designed to detect only the plasmid-borne FKS2 promoter. PCRs from whole-cell extracts (WCE) are also shown. (B) ChIP analysis of the FKS2 promoter with Mlp1-HA. Wild-type yeast strain 1788 was cotransformed with FKS2-lacZ reporter plasmids from panel A and multicopy plasmids expressing the indicated HA-tagged MLP1 allele (wild-type, p2022; mlp1-Y192F, p2024; or vector, YEp351). Transformants were cultivated and subjected to thermal stress prior to ChIP analysis, as above. WT, wild type; TAYF, T190A Y192F.