TABLE 2.
Plasmids
| Plasmid | Description | Reference or source |
|---|---|---|
| pRS315 | LEU2-based centromeric plasmid | 60 |
| YEp351 | LEU2-based 2μm plasmid | 31 |
| YEp352 | URA3-based 2μm plasmid | 31 |
| p740 | YEp351[MPK1] | 37 |
| p777 | YEp351[MPK1-3xHA] | 37 |
| p778 | YEp351[mpk1(T190 Y192F)-3xHA] | 37 |
| p903 | pLGΔ-312; CYC1-lacZ reporter vector | 26 |
| p904 | pLGΔ-178; CYC1-lacZ reporter vector | 26 |
| p916 | FKS2(−706 to −360)-CYC1-lacZ in p903 | 70 |
| p950 | YEp352[FKS2] | 70 |
| p1070 | FKS2(−706 to −360)-CYC1-lacZ with LEU2 marker; derived from p916 | This study |
| p1150 | YEp24(SWI4) | Joseph Gray |
| p1172 | pGBT9; 2-hybrid vector with Gal4 DBD | Clontech |
| p1173 | pGAD424; 2-hybrid vector with Gal4 AD | Clontech |
| p1366 | PRM5(YIL117c)-lacZ | 35 |
| p2022 | YEp351[MLP1-3xHA] | This study |
| p2024 | YEp351[mlp1(Y192F)-3xHA] | This study |
| p2052 | FKS2(−540 to −375)-CYC1-lacZ with URA3 marker | This study |
| p2053 | FKS2(−540 to −375)-CYC1-lacZ with SBF-binding site mutation at −386T | This study |
| p2054 | FKS2(−540 to −375)-CYC1-lacZ with SBF-binding site mutation at −388C | This study |
| p2055 | FKS2(−540 to −375)-CYC1-lacZ with SBF-binding site mutation at −389G | This study |
| p2066 | CLN2(−600 to −400)-CYC1-lacZ | This study |
| p2119 | YEp351[mpk1(K54R)-3xHA] | This study |
| p2188 | pRS315[MPK1-3xHA] | This study |
| p2190 | pRS315[mpk1(T190A Y192F)-3xHA] | This study |
| p2193 | pRS315[mpk1(K54R)-3xHA] | This study |
| p2248 | pGBT9 MPK1(1-328) | 62 |
| p2313 | YEp351[MPK1-FLAG] | 40 |
| p2316 | YEp351[mpk1(T190A Y192F)-FLAG] | This study |
| p2317 | YEp351[mpk1(K54R)-FLAG] | This study |
| p2339 | BG1805-GAL1-SWI4-ZZ-HA-6HIS | 24 |
| p2341 | BG1805-GAL1-SWI6-ZZ-HA-6HIS | 24 |
| p2344 | pRS304[swi4Δ::TRP1] | This study |
| p2345 | pRS305[swi6Δ::LEU2] | This study |
| p2346 | pRS315[MLP1-3xHA] | This study |
| p2347 | pRS315[mlp1(Y192F)-3xHA] | This study |
| p2348 | pUT34; HIS3-based MET25 promoter | 49 |
| p2349 | pUT34[ERK5-6HIS] | This study |
| p2350 | pUT34[ERK5(T219A Y221F)-6HIS] | This study |
| p2351 | pUT34[ERK5(K84R)-6HIS] | This study |
| p2352 | pGAD424 SWI4(1-1093) | This study |
| p2415 | pUT36; URA3-based MET25 promoter | 49 |
| p2418 | pUT36 (SWI4-6HIS) | This study |