TABLE 1.
Description of p53 mutants useda
| p53 mutation | Mutation type | Species | Selection/vector |
|---|---|---|---|
| 143A | Class II (conformation) | Human | Puromycin/ pBabe-puro |
| 175H | Class II (conformation) | Human | G418/LXSN |
| 248W | Class I (DNA contact) | Human | G418/LXSN |
| Residues 275-368 | GSE | Rat | G418/LXSN |
a
All p53 genes used were full-length human cDNAs, with the exception of GSE, which corresponds to a 94-amino-acid peptide derived from rat p53 (38). This collection of p53 alleles includes mutants of all three classes of mutation (see text). Drug selections were as indicated.