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. 2008 Feb 19;28(8):2825–2839. doi: 10.1128/MCB.02076-07

FIG. 1.

FIG. 1.

Human DOT1L occupies transcribed regions in a ubiquitous fashion. (A) ChIP in HEK293T cells transfected with pFLAG-DOT1L or empty vector. (B, D, F, and G) ChIP with the indicated antibodies in HEK293T cells across the PABPC1 gene with PCR primers at the indicated locations relative to the TSS. CD4 is a negative control region. (C and E) ChIP-qPCR analysis of HeLa cells at genes with high or low expression with the indicated antibodies. The DOT1L antibody is A300-954A. Primers amplify within the +0.5-kb region relative to the TSS. IgG, immunoglobulin G.