FIG. 3.

Adr1 binding and activation in Mediator mutants. (A) Beta-galactosidase assays of strains TYY497 (wild type [WT]), TYY540 (med15Δ), TYY541 (med2Δ), TYY542 (med3Δ), and TYY543 (med16Δ), all with CEN-TRP1 plasmid pKD16, which contains the ADR1 gene with a C-terminal HA tag. Cultures were assayed under repressing conditions (R) or after 6 or 24 h of derepression (DR). (B) ChIP for Adr1 in the wild-type and med15Δ strains used for panel A under repressing conditions (R) or after 1 or 2 h of derepression (DR). Data are expressed as increases over background from an unbound telomeric (TEL) region. Error bars show standard deviations from triplicate measurements of each sample.