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Adr1-coactivator activation of ADH2 expression measured by real-time qRT-PCR
| Strain | Activator |
ADH2 mRNA level/ACT1 mRNA levela
|
|
|---|---|---|---|
| Repressedb | Derepressedc | ||
| TYY497 | None | 0.025 (0.0015) | NAd |
| Wild-type Adr1 | 0.012 (0.01) | 1.45 (0.08) | |
| Adr1-Med15 | 0.92 (0.09) | NA | |
| CTYTY61 | Wild-type Adr1 | NA | 1.3 |
| ECY10 | Med3-Adr1 | 1.9 | 15.5 |
| ECY1 | None | 0.002 | 0.012 |
| Adr1-TBP | 0.030 | 2.0 | |
Standard deviations, when measured, are given in parentheses.
5% glucose.
For 4 to 6 h (0.05% glucose).
NA, not assayed.
