TABLE 4.
Adr1-Med15 activation depends on the Mediator tail
| Strain | Activator | Mediator mutation | β-Gal activitya
|
ADH2 mRNA level/ACT1 mRNA levelb
|
||
|---|---|---|---|---|---|---|
| Repressed conditions | Derepressed conditions | Repressed conditions | Derepressed conditions | |||
| TYY204 | Adr1-Med15 | None (WT) | 260 (100) | 1,400 (100) | ||
| TYY540 | Adr1-Med15 | med15 (gal11)Δ | 400 (150) | 1,200 (86) | ||
| TYY541 | Adr1-Med15 | med2Δ | 36 (14) | 250 (18) | ||
| TYY542 | Adr1-Med15 | med3 (pgd1)Δ | 18 (6.9) | 410 (29) | ||
| TYY543 | Adr1-Med15 | med16 (sin4)Δ | 120 (46) | 210 (15) | ||
| TYY309 | Adr1 | None (WT) | 0.002 | 7.1 | ||
| TYY309 | Adr1-Med15 | None (WT) | 0.92 | 13 | ||
| TYY317 | Adr1-Med15 | gcn5Δ | 0.90 | 1.5 | ||
a
Values are expressed as Miller units, with percentages of β-galactosidase activity of the wild-type Mediator shown in parentheses. The standard deviation of triplicate assays was about 20%. Derepression, overnight in 0.05% glucose.
b
Derepression, 14 h in 0.05% glucose.