TABLE 5.

Snf1-independent activation by artificial recruitment of Mediator

Gene	Growth conditiona	Relative expression level with activatorb:
		Med3-Adr1 DBDc		Adr1 WT
		SNF1	snf1	SNF1	snf1
ADH2	R	120	100	0.5	0.5
	DR	1,700	300	126	0.4
ATO3	R	41	67	6.3	3.4
	DR	266	150	50	4.5
ALD4	R	4.0	3.5	0.1	0.0
	DR	110	33	9.7	0.2
ACS1	R	20	6.0	1.1	0.9
	DR	950	120	190	3.9
FDH	R	0.2	0.2	0.2	0.2
	DR	56	4.6	2.3	0.4
ADY2	R	1.5	1.6	0.5	0.4
	DR	2,900	100	240	0.7
ICL2	R	0.9	0.8	0.9	0.7
	DR	18	0.9	7.3	1.2
FBP1	R	0.2	0.2	0.2	0.1
	DR	17	0.3	130	0.6
ICL1	R	1.5	1.7	1.1	0.8
	DR	4.9	1.5	87	1.4
MLS1	R	0.3	0.2	0.2	0.2
	DR	12	0.4	87	0.4
MDH2	R	4.3	3.0	1.9	1.5
	DR	58	12	43	3.9

^aR, repression (5% glucose); DR, derepression for 6 h (0.05% glucose).

^bmRNA levels were calculated by qRT-PCR as described in Materials and Methods. The quantities determined for each primer pair were divided by the quantity determined for the control ACT1. WT, wild type.

^cStrains were ECY10, CTYTY75, CTYTY61, and CTYTY66.