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. 2008 Feb 11;28(8):2637–2647. doi: 10.1128/MCB.01601-07

FIG. 1.

FIG. 1.

Homer 1 colocalizes with alpha-actinin at the Z-disk. (A) Immunostaining of alpha-actinin in adult WT plantaris muscle. (B) Immunostaining of adult WT plantaris muscle using a pan-Homer Ab. (C) Merged image of panels A and B shows that Homer proteins and alpha-actinin colocalize. (D) Immunostaining of alpha-actinin in Homer 1 KO skeletal muscle. (E) Immunostaining of Homer 1 KO skeletal muscle using a pan-Homer Ab. (F) Western blotting using a pan-Homer Ab shows Homer expression in predominantly fast-twitch (plantaris) and predominantly slow-twitch (soleus) skeletal muscles from WT and Homer 1 KO mice. Non-Homer 1 isoforms are expressed in soleus muscle. (G) RT-PCR of cDNA generated from plantaris muscle shows the expression of coiled-coil Homer 1 isoforms, Homer 1b and Homer 1c, in adult mouse skeletal muscle. (H) DAPI (4′,6′-diamidino-2-phenylindole [blue]) and dystrophin (red) immunostaining of WT and Homer 1 KO mouse muscle. (I) Cross-sectional area of myofibers from muscles of Homer 1 KO (H1KO) mice (n = 1247) compared with WT littermates (n = 1343). Fiber cross-sectional area was 42.1 × 102 ± 80 μm2 in Homer 1 KO mice versus 53.6 × 102 ± 65 μm2 in WT littermate controls (mean ± SE; P < 0.05). (J) Distribution of muscle fiber cross-sectional areas from WT (blue) and Homer 1 KO mice (red).