FIG. 2.

Chromatin structure of SNR6 under repression. Nutrient deprivation in 0.15× YEP medium lacking glucose was used to repress Pol III transcription. Occupancies were calculated as relative enrichment over the TEL VIR region, and the averages from three independent experiments, with error bars, are shown. (A) ChIP assays showing occupancy of the HA-tagged RPC160 over SNR6 in wild-type cells without (active) or with repression for 40 min (repressed). (B) Progressive increase in occupancy of the FLAG-H2B over TATA-A box region under repressed condition. (C) In vivo IEL analysis of chromatin before (lane 1) and after shifting the cells to 0.15× YEP medium for 1 h (lanes 2 to 4). Lane A, control sample under active state without repression. Gray ovals denote the position of a nucleosomal protection region. Arrowheads with numbers indicate the positions of MNase cut sites.