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Calibration of excitation ratios as a function of pHi of E. coli and L. innocua cells in buffers from pH 5.5 to pH 8.0. The GFP-transformed E. coli cells were pH equilibrated by using CCCP and an excitation ratio of 470 nm/410 nm. The CFDA-SE-stained L. innocua cells were pH equilibrated with ethanol and an excitation ratio of 490 nm/435 nm. Error bars show SEMs.
