TABLE 2.

τ_1/2 of trapped cells

Species	Shaking during growtha	Fluorophore	Laser power (mW)	Initial pHi	τ1/2 (min)b
E. coli	+	GFP	6	7.8	»60
E. coli	+	GFP	18	7.8	30
L. monocytogenes	+	GFP	6	7.3	»60
L. monocytogenes	+	CFDA-SE	6	7.7	»60
L. monocytogenes	−	CFDA-SE	6	7.6	57
L. innocua	+	CFDA-SE	6	7.6	»60
L. innocua	−	CFDA-SE	6	7.4	10
B. subtilis	+	CFDA-SE	6	7.2	21

^a+, with shaking; −, without shaking.

^bWhen the pH gradient is not halved within 60 min, τ_1/2 is designated as »60 min.