TABLE 2.
PCR primer sequences targeting gyrB used for Campylobacter species-specific identification
| Strain | Forward primer | Reverse primer | Amplicon length (bp) | Location |
|---|---|---|---|---|
| C. jejuni NADC 5096 | AGAATGGGTTTAACTCGTGTGATAAGT | TACCACGCAAAGGCAGTATAGCT | 493 | 84-576 |
| C. coli NADC 5095 | AAATGCTAGTGCTAGGGAAAAAGACTCT | TGAGGTTCAGGCACTTTTACACTTACTAC | 96 | 125-220 |
| C. concisus ATCC 33237 | AGCGGGCCTAACAAGAGTTATTACA | TGTAAGCACGTCAAAAACCATCTTT | 217 | 86-302 |
| C. curvus ATCC 35224 | CTGCCAAAGTAAGGACGCAAGTATA | GGCAAGATCGCCTGAAATACG | 108 | 458-565 |
| C. showae ATCC 51146 | AGGGTTTAAGCATAGGAACGCTG | CACCAGATAAAGCTCGCTGATCG | 86 | 415-500 |
| C. mucosalis ATCC 49352 | TGCGATTATGAACAAGGCCCTA | TCGCTTGAAACACACGGTCA | 224 | 335-558 |
| C. fetus subsp. fetus ATCC 15296 | AGAGCTGGGCTTACAAGAGCTATTACA | GGTAAAATCGCTTGAAACGCTCTAT | 482 | 84-565 |
| C. hyointestinalis ATCC 35217 | CGGTCAAAAGATGACTTTTGAAGTACTT | GCTTCCCTGCCACGAGCT | 108 | 272-379 |
| C. sputorum subsp. sputorum ATCC 33562 | AGCTTTACTTGCTGCAAGAGGAAGA | AGGAAGCGTTCCAACAGAAAAGTT | 94 | 350-443 |
| C. helveticus ATCC 51210 | CAATAACATACGCACACCAGATGGA | CAGGCACTTTAACGCTCACTATGG | 176 | 38-213 |
| C. upsaliensis ATCC 49816 | GCTTACGCGTGTAATTACAAACTATGTC | AATTGCCTTAGCCTCGATAGGG | 250 | 92-341 |
| C. lari ATCC 35221 | CTATGTTCGTCCTATAGTTTCTAAGGCTTC | CCAGCACTATCACCCTCAACTAAATAA | 261 | 257-517 |