FIG. 1.
Formation of aldehydes and clearing zones on latex overlay-agar plates. Recombinant strains of S. lividans strain TK23 harboring plasmid pIJSK::lcpK30 (A), pIJSK::lcpVH2 (B), pIJSK::lcpKb1 (C), or pIJSK (D) were incubated for 10 days at 30°C on latex overlay-agar plates containing 0.5% (wt/vol) glucose and stained with Schiff's reagent to detect aldehydes; results are shown in the upper part of the figure. Clearing-zone formation was documented for the same recombinant strains of S. lividans strain TK23 in the corresponding panels at the bottom of the figure after 30 days of incubation at 30°C on latex overlay-agar plates containing 0.5% (wt/vol) glucose. Thiostrepton (25 μg/ml) was added to the medium for plasmid maintenance.