FIG. 2.

Change in the abundances of Lactobacillus and Gardnerella rRNA genes during linear amplification with three different 27f primer formulations. Amplification was carried out with a human vaginal DNA sample at the following three different annealing temperatures: 48°C, 54°C, and 60°C. Each sample was analyzed by qPCR using primers specific for Lactobacillus (left panels) and Gardnerella (right panels). Data are relative fluorescence units of DNA in the 26th cycle of qPCR. The fluorescence units are normalized to “two strands” at zero cycles of linear amplification. Each data point represents the average of results from three independent experiments.