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Change in Lactobacillus and Gardnerella sequence ratios after PCR amplification using each of three 27f primer formulations and the 1492r primer. The values plotted are ΔΔCTs, the change in the Lactobacillus CT minus the Gardnerella CT resulting from 23 cycles of PCR amplification of the DNA samples. Values are the means and the corresponding standard errors from three experiments based on the same data as are shown in Table 2.
