TABLE 1.
PCR and RT−RT−PCR primers used in this study
| Primer name | Gene(s) | Gene ID(s) | Sequence |
|---|---|---|---|
| PCR | |||
| UreaseForw | ureABC | BCE3662−BCE3664 | ATCAGATATTCAAGTCGAGG |
| UreaseRev | ureABC | BCE3662−BCE3664 | CCAGGTGTTATTGTAGTTGC |
| RT−RT−PCR | |||
| QPCR_ureA_Forw | ureA | BCE3664 | TTCCGCCACATTTTTACCATC |
| QPCR_ureA_Rev | ureA | BCE3664 | ACGAAAGGAGAGGGGGCTTA |
| QPCR_ureG_Forw | ureG | BCE3659 | GCCCCTACATAAGGTGCCAAA |
| QPCR_ureG_Rev | ureG | BCE3659 | GCGCAGGGAGAAAAGATTCC |
| QPCR_ureI_Forw | ureI | BCE3657 | GGAATGCCCAAAAGAACCAA |
| QPCR_ureI_Rev | ureI | BCE3657 | CATTGCAACGGTTATGGGAAT |
| QPCR_nikT_Forw | nikT | BCE3656 | CCATGTTTTGCCGTTTTTCC |
| QPCR_nikT_Rev | nikT | BCE3656 | ACTCACAGGGGGTGGGATTT |
| QPCR_rpoA_Forw | rpoA | BCE0137 | GCCCAGGTCACGCTGACTAT |
| QPCR_rpoA_Rev | rpoA | BCE0137 | TCACGTGTTTGAGGCATTGG |
| QPCR_tufA_Forw | tufA | BCE0108 | ACCGCTTGAGCGTGTGGATATG |
| QPCR_tufA_Rev | tufA | BCE0108 | TAGCAGTAACAGCGGCACCA |