TABLE 1.
Distribution of 14C residues from [14C]diuron in agricultural soil inoculated with Variovorax sp. strain SRS16 or A. globiformis D47 or with an SRS16-D47 coculture as estimated by thin-layer chromatography and autoradiographic quantification, including detection of the demethylated diuron metabolite DCPMU, the didemethylated metabolite DCPU, and 3,4-DCA
| Prepn |
14C recovery (%)a
|
||||||
|---|---|---|---|---|---|---|---|
| [14C]diuron | [14C]DCPMU | [14C]DCPU | [14C]3,4-DCA | 14CO2 | 14C-labeled humic fraction | Nonextractable 14C | |
| Soil + SRS16 | 23.1 (5.5) | 1.8 (0.5) | 1.5 (0.1) | 0.0 | 36.6 (4.1) | 5.4 (0.4) | 31.5 (1.5) |
| Soil + D47 | 16.6 (3.2) | 1.6 (0.1) | 1.7 (0.3) | 3.9 (1.5) | 5.9 (0.5) | 14.9 (0.8) | 55.4 (3.2) |
| Soil + SRS16 + D47 | 1.1 (0.6) | 0.2 (0.1) | 0.6 (0.2) | 0.0 | 59.9 (2.3) | 4.4 (0.5) | 33.7 (0.9) |
| Soil | 78.8 (0.9) | 3.0 (0.5) | 1.7 (0.2) | 0.0 | 1.2 (0.0) | 0.0 | 15.3 (1.2) |
a
The values are means and standard deviations (in parentheses) for triplicate experiments expressed as percentages of the initial amount of [14C]diuron.