FIG. 1.

Physiological response of strain EbN1 to solvent stress during anaerobic growth with ethylbenzene (A and B) and succinate (C and D). (A) Growth of ethylbenzene-adapted cells with 0.08 mM ▵, 0.21 mM □, and 0.32 mM ⧫ ethylbenzene. These values represent the equilibrium concentrations in the aqueous medium when the ethylbenzene was supplied in an inert carrier phase. (C) Growth of succinate-utilizing cells suddenly exposed (indicated by an arrow) to a solvent mixture (containing ethylbenzene, toluene, p-cresol, and phenol) having a total solvent concentration of 0 mM ▵, 2.7 mM ⧫, or 3.0 mM □ in the culture medium. The vertical dotted lines indicate time points when mass cultures were harvested for proteomic studies (45 or 250 min after solvent addition). Concentrations of nitrate (solid lines) and nitrite (dashed lines) are indicated in panels B and D.