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c-di-GMP is epistatic to QS in the control of biofilms. Biofilm formation in overnight static cultures of V. cholerae WT and luxO(D47E) strains containing either the vector alone (denoted vector) or overexpressing QrgB, QrgB* (GGEEF→AAEEF), VC1086, or VC1086* (EAL→AAL).