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HapR binds the vpsT promoter. Gel mobility shift analyses of the vpsT, vpsR, and vpsL promoters with purified HapR. HapR amounts are as follows: lanes 1 and 6, 0 nM; 2 and 7, 40 nM; 3 and 8, 120 nM; 4 and 9, 250 nM; and 5 and 10, 500 nM. aphA−, no HapR binding site; aphA+, contains the HapR binding site.
