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Amount of taurocholate required for efficient recovery of C. difficile spores. C. difficile spores were incubated in water containing increasing concentrations of taurocholate, serially diluted, and plated on BHIS agar in the absence of taurocholate. Colonies were enumerated after overnight growth, and data were compared to those for spores spread on BHIS(TA). Data points are means from three independent experiments, and error bars represent 1 standard deviation from the mean.
