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. 2008 Feb 1;190(7):2505–2512. doi: 10.1128/JB.01765-07

FIG. 4.

FIG. 4.

Effect of primary bile salts on the germination and growth of C. difficile. (A) C. difficile spores were purified and suspended in BHIS alone (•), in 1% taurocholate in buffer (▾), or in BHIS containing 1% taurocholate (⧫), 1% glycocholate (▴), or 1% cholate (▪). The ratios of the optical densities at various time points to the starting optical density are plotted against time. (B) C. difficile CD196 spores were purified and suspended in buffered glycine (1.3 mM) (▴), buffered 1% taurocholate (▾), or buffered glycine plus taurocholate (⧫). Germination was measured as for panel A. (C) C. difficile UK14 spores were purified and suspended in buffered glycine (1.3 mM) (▴), buffered 1% taurocholate (▾), or buffered glycine plus taurocholate (⧫). Germination was measured as for panel A. (D) Vegetative C. difficile (solid lines) and vegetative C. perfringens (dashed lines) were grown in BHIS alone (•), BHIS(TA) (⧫), or BHIS plus 0.1% chenodeoxycholate (×). Error bars represent 1 standard deviation from the mean.