Strains |
|
|
DK1622 |
wild type |
20 |
DK1622GFP |
Wild type, attB::pilA-gfp
|
18 |
DK10409 |
ΔpilT
|
59 |
DK10410 |
ΔpilA
|
59 |
DK10416 |
ΔpilB
|
59 |
SA2020 |
ΔpilT attB::pSL104 Kmr
|
This work |
SA2021 |
ΔpilB attB::pSL105 Kmr
|
This work |
SA2412 |
ΔpilT attB::pSL104TWalkerA Kmr
|
This work |
SA2413 |
ΔpilT attB::pSL104TWalkerB Kmr
|
This work |
SA2414 |
ΔpilT attB::pSL105BWalkerA Kmr
|
This work |
SA2415 |
ΔpilT attB::pSL105BWalkerB Kmr
|
This work |
Plasmids |
|
|
pSW105 |
Mx8 attP, pilA promoter, Kmr
|
S. Weiss |
pMS421 |
lacIq Strr
|
11 |
pUHE24-2 |
PA1/O4/O3, Ampr
|
24 |
pSL104 |
pSW105 with pilT
|
This work |
pSL104TWalkerA |
pSL104 with Walker A box mutation in pilT
|
This work |
pSL104TWalkerB |
pSL104 with Walker B box mutation in pilT
|
This work |
pSL105 |
pSW105 with pilB
|
This work |
pSL105BWalkerA |
pSL105 with Walker B box mutation in pilB
|
This work |
pSL105BWalkerB |
pSL104 with Walker B box mutation in pilB
|
This work |
pSL4 |
pUHE24-2 with His6-pilT
|
This work |
pSL4TWalkerA |
pSL4 with Walker A box mutation in pilT
|
This work |
pSL4TWalkerB |
pSL4 with Walker B box mutation in pilT
|
This work |
pSL5 |
pUHE24-2 with His6-pilB
|
This work |
pSL5BWalkerA |
pSL5 with Walker A box mutation in pilB
|
This work |
pSL5BWalkerB |
pSL5 with Walker B box mutation in pilB
|
This work |