TABLE 1.
M. xanthus strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| DK1622 | wild type | 20 |
| DK1622GFP | Wild type, attB::pilA-gfp | 18 |
| DK10409 | ΔpilT | 59 |
| DK10410 | ΔpilA | 59 |
| DK10416 | ΔpilB | 59 |
| SA2020 | ΔpilT attB::pSL104 Kmr | This work |
| SA2021 | ΔpilB attB::pSL105 Kmr | This work |
| SA2412 | ΔpilT attB::pSL104TWalkerA Kmr | This work |
| SA2413 | ΔpilT attB::pSL104TWalkerB Kmr | This work |
| SA2414 | ΔpilT attB::pSL105BWalkerA Kmr | This work |
| SA2415 | ΔpilT attB::pSL105BWalkerB Kmr | This work |
| Plasmids | ||
| pSW105 | Mx8 attP, pilA promoter, Kmr | S. Weiss |
| pMS421 | lacIq Strr | 11 |
| pUHE24-2 | PA1/O4/O3, Ampr | 24 |
| pSL104 | pSW105 with pilT | This work |
| pSL104TWalkerA | pSL104 with Walker A box mutation in pilT | This work |
| pSL104TWalkerB | pSL104 with Walker B box mutation in pilT | This work |
| pSL105 | pSW105 with pilB | This work |
| pSL105BWalkerA | pSL105 with Walker B box mutation in pilB | This work |
| pSL105BWalkerB | pSL104 with Walker B box mutation in pilB | This work |
| pSL4 | pUHE24-2 with His6-pilT | This work |
| pSL4TWalkerA | pSL4 with Walker A box mutation in pilT | This work |
| pSL4TWalkerB | pSL4 with Walker B box mutation in pilT | This work |
| pSL5 | pUHE24-2 with His6-pilB | This work |
| pSL5BWalkerA | pSL5 with Walker A box mutation in pilB | This work |
| pSL5BWalkerB | pSL5 with Walker B box mutation in pilB | This work |