TABLE 2.
T4P formation in M. xanthus mutants carrying substitutions in the Walker A and Walker B boxes of PilB and PilT
| Strain | Genotype | No. of cells
|
No. of pili per cell | |||
|---|---|---|---|---|---|---|
| Total scored | Nonpiliated | Piliated at one pole | Piliated at both poles | |||
| DK1622 | Wild type | 21 | 6 | 15 | 0 | 9 |
| DK10410 | ΔpilA | 21 | 21 | 0 | 0 | 0 |
| DK10416 | ΔpilB | 20 | 20 | 0 | 0 | 0 |
| SA2021 | ΔpilB/pilB+ | 20 | 2 | 18 | 0 | 7 |
| SA2414 | ΔpilB/pilB(K328A) | 20 | 20 | 0 | 0 | 0 |
| SA2415 | ΔpilB/pilB(E392A) | 20 | 20 | 0 | 0 | 0 |
| DK10409 | ΔpilT | 21 | 2 | 19 | 0 | 7 |
| SA2020 | ΔpilT/pilT+ | 21 | 2 | 19 | 0 | 10 |
| SA2412 | ΔpilT/pilT(K138A) | 21 | 3 | 18 | 0 | 7 |
| SA2413 | ΔpilT/pilT(E206A) | 21 | 5 | 16 | 0 | 9 |