TABLE 1.

Primers used for construction of deletion mutations in ECF sigma genes

Primer set	Sequencea
	F	R
sigM5′	5′ GGAGGATCCCTTCAATTGGCACAAGCG	5′ CAGCCGCTGTTAATCGATCGTCACTGCCTT
sigM3′	5′ ACGATCGATTAACAGCGGCTGAAAGCACTT	5′ GGAGGATCCGCTTTTGCGGTCGTTTGT
sigV5′	5′ GAAGAATTCAGGAGATAGAAGCAGAGC	5′ CGTCAACTGTTATTTCTTCATTGCAATAAAGGG
sigV3′	5′ ATGAAGAAATAACAGTTGACGAAGGAGGAT	5′ GGAGGATCCGACTTTGATATAGCGCTC
sigW5′	5′ GAAGAATTCTAACACGGGTTCGAATCC	5′ AAGATCCCTTTAAATCATCATTTCCATATTTATCTA
sigW3′	5′ ATGATGATTTAAAGGGATCTTTAAGTGGGG	5′ GGAGGATCCTCCCCGCAGACTTCATCT
sigX5′	5′ GAAGAATTCAAGTTTTCCGGTGTTGCG	5′ CCTCAAAAGTTAGGTTTCTTCCATTTGAAACCC
sigX3′	5′ GAAGAAACCTAACTTTTGAGGGAGGAGCTA	5′ GGAGGATCCCCGAACCCCAGTCTCATT
sigY5′	5′ GAAGAATTCGATCCCGGGATGGGAACA	5′ CTTTCTGATTTATTGTGTATCCAATGACCG
sigY3′	5′ GATACACAATAAATCAGAAAGGAGTGGGAT	5′ GGAGGATCCCGTTCGTCCGCTCTTCTT
sigZ5′	5′ GAAGAATTCAGTCTACTTTGCCGGAGC	5′ ATCCGCTTCTTATTGATCCCATAGATCCTC
sigZ3′	5′ TGGGATCAATAAGAAGCGGATCGATATGGA	5′ GGAGGATCCCGTATCCGTCATACGTAT
ylaC5′	5′ GAAGAATTCGCAGTCAGGGTGACATGG	5′ CTCTCTACTTTAGGAATCCCTATGCTTCAT
ylaC3′	5′ AGGGATTCCTAAAGTAGAGAGGAGGAGCGG	5′ GGAGGATCCGCAGTTGAACTTGGCTTC

^aEcoRI and BamHI sites are underlined. Boldface letters indicate TAA stop codons.