TABLE 1.
Primers used
| Primer | Sequence (5′ to 3′)a |
|---|---|
| GP-IF | TGTGGCCAATGAGCAGTGGG |
| GP-IR | ACCGGGTGATTTCCCAGACC |
| GP-RF | GGATATCCAACGGAATGGGTC |
| GP-RR | GTCAAAGTTAACGGTTCGCTC |
| A-F | CCGGAATTCACATGATGTCGCTTCTGCCG |
| A-R | CCCAAGCTTCTGCGATGGAGAGCGAGTTC |
| S1-IF | CCGGAATTCGGATATGTCGCTCCCCATTC |
| S1-IR | GGGGACTTCCCACTGCTCATTAG |
| S1-RF | TCCGCAATAGCCTGCCTCTC |
| S1-RR | GTGGCTGCGTTCTCAAGGTC |
| UF | TCCGCAATAGCCTGCCTCTC |
| RF | GACCTTGAGAACGCAGCCAC |
| IF | CCGATATGATGCCCGATCCG |
| AF | CGGAGAACTCGCTCTCCATC |
| RR | ATCAGCCCTGCGCAGATAGC |
| IR | TGCATGCTGCTGCTCAATGC |
| AR | GGTGCCAGCCTGATAATTGC |
| DR | ACCAGGTGCGTGAGGGCATG |
a
Restriction sites are underlined.