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. 2008 Jan 25;190(7):2279–2285. doi: 10.1128/JB.01705-07

FIG. 3.

FIG. 3.

Amino terminus of Rns is essential for positive autoregulation, activation of the CFA/I pilus promoter, and repression of nlpAp. (A) Western blot probed with a polyclonal antibody against the hexahistidyl epitope tag, demonstrating expression of the fusion proteins His6-Rns(61-265), His6-Rns(100-265), and His6-Rns(117-265). The arrowheads indicate the positions of the 25-, 20-, and 18-kDa fusion proteins. (B) β-Galactosidase activity (n ≥ 3) from promoter-lacZ fusions integrated into the chromosome of K-12 strain MC4100.