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Effects of mutations near the amino terminus of Rns vary depending on the promoter: β-galactosidase activity (n ≥ 3) from promoter-lacZ fusions integrated into the chromosome of K-12 strain MC4100. For the Rns-activated promoters rnsp, cfaAp, and cotBp, activity is expressed relative to the activity of an isogenic strain with wild-type Rns. For nlpAp, which is repressed by Rns, activity is expressed relative to the activity of an isogenic strain lacking Rns.
