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SDS-PAGE and Western blotting of P. gingivalis W50 total LPS and pure A-LPS. (A) SDS-urea-PAGE. (B) Western blotting versus MAb 1B5 of P. gingivalis W50 total LPS prepared using the phenol-guanidine isothiocyanate method and A-LPS purified by ConA affinity chromatography. Lanes: 1 and 3, W50 total LPS; 2 and 4, pure A-LPS.
