. 2007 Dec 7;190(8):2700–2708. doi: 10.1128/JB.01620-07

TABLE 1.

Strains and plasmids

Strain or plasmid	Relevant genotype or characteristics^a	Source or reference
E. coli strains
XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI^qZΔM15 Tn10(Tc^r)]	Stratagene
S17.1	thi pro hsdR recA RP4-2 (Tc::Mu) (Km::Tn7)	39
P. aeruginosa strains
PAO1	Wild type	42
PA103	Wild type	22
PA103 pilA	PA103 ΔpilA::Gm^r; Gm^r	47
pilA	PAO1 ΔpilA	6
fliC	PAO1 ΔfliC::Tc^r; Tc^r	6
fliC pilA	PAO1 ΔfliC::Tc^r ΔpilA; Tc^r	6
fliC pilA	PAO1 ΔfliCΔpilA	This work
fliC pilA gacA	PAO1 ΔfliC::Tc^r ΔpilA gacA::Tn<Gm^r>; Tc^r Gm^r	This work
fliC pilA gacA	PAO1 ΔfliCΔpilA gacA::Tn<Gm^r>; Gm^r	This work
fliC pilA gacS	PAO1 ΔfliC::Tc^r ΔpilA gacS::Tn<Gm^r>; Tc^r Gm^r	This work
fliC pilA retS	PAO1 ΔfliC::Tc^r ΔpilA retS::Tn<Gm^r>; Tc^r Gm^r	This work
fliC pilA gacA attB::pilA	PAO1 ΔfliC ΔpilA gacA::Tn<Gm^r> attB::pilA; Gm^r	This work
fliC pilA rhlA	PAO1 ΔfliC::Tc^r ΔpilA ΔrhlA; Tc^r	This work
Plasmids
pX1918G	Cloning/expression vector; Gm^r	36
pMOD2<MCS>		Epicentre
pMOD<Gm^r>	aacC1-xylE cassette cloned at MCS of pMOD2<MCS>	This work
mini-CTX2	Contains attP site for integration at P. aeruginosa chromosomal attB site; Tc^r	13
mini-CTX-pilA	pilA under control of its own promoter in mini-CTX2	This work
pUCP-KS	Shuttle vector; Ap^r Cb^r	46
pRhlA	rhlA under control of the P_lac promoter in pUCP-SK; Ap^r Cb^r	This work
pEX18 Gm	Suicide vector; Gm^r; sacB	13
pEX18-ΔpilA	pilA knockout construct in pEX18 Gm; Gm^r; sacB	6
pEX18-ΔfliC	fliC knockout construct in pEX18 Gm; Gm^r; sacB	This work
pMQ30	Suicide vector; sacB; Gm^r URA3 CEN6/ARSH4	37
pKO-rhlA	rhlA knockout construct in pMQ30; Gm^r; sacB	This work
pMQ80	Cloning vector; Gm^r URA3 P_BAD; araC	37
pBifA	His-tagged bifA under control of the P_BAD promoter in pMQ80; Gm^r URA3	18
pSadC	C-terminal HA-tagged sadC under control of the P_BAD promoter in pMQ80; Gm^r URA3	26

Abbreviations: Ap, ampicillin; Cb, carbenicillin; Gm, gentamicin; Tc, tetracycline; Km, kanamycin.