FIG. 7.

Fork reversal activity of StoHjm in the presence of StoHjc. (A) Cleavage by StoHjc (50 nM) in association with StoHjm (64 nM) and in the presence of ATP (5 mM). Both nascent strands were labeled, while the parent strands were not labeled. Labeled 48-, 36-, 26-, and 20-mer strands were used as markers. (B) Cleavages observed when substrates with each of the four strands labeled were used. The substrates used are shown above lanes 1 to 8. The substrates with either nascent strand labeled were used as controls (lanes 9 and 10). StoHjm (100 nM) was used in the reactions. (C) Schematic illustration of reactions shown in panels A and B.