TABLE 1.
Genes identified as genes that are significantly up- or downregulated in medium containing 50 or 500 μM CuCl2 compared with Cu-free medium
| Category and genea | Change (fold) with 50 μM Cu | Change (fold) with 500 μM Cu | Putative function | Oxidative stress |
|---|---|---|---|---|
| Regulators | ||||
| Rv0967 (csoR) | 4.3 | 30.8 | Copper-sensitive repressorb | |
| Rv1909c (furA) | 3.8 | Ferric uptake regulation protein | Yes | |
| Rv1994c | 4.0 | Probable MerR transcriptional regulatory proteinb | Yes | |
| Rv2642 | 3.9 | Probable ArsR family transcriptional regulatory protein | ||
| Transporters | ||||
| Rv0969 (ctpV) | 1.7 | 2.9 | Probable metal cation transporter P-type ATPase | |
| Rv0849 | 4.4 | Probable permease | Yes | |
| Rv2398c (cysW) | 3.8 | Probable sulfate transport ABC transporter | ||
| Rv2963 | 2.6 | 15.7 | Possible permeaseb | Yes |
| Membrane protein | ||||
| Rv0970 | 3.2 | Probable integral membrane protein | ||
| Transposases | ||||
| Rv1765A | −3.3 | −2.8 | Putative transposase (fragment) | |
| Rv0850 | 6.4 | Putative transposase (fragment) | Yes | |
| Enzymes | ||||
| Rv0815c (cysA2) | 3.4 | Probable thiosulfate sulfurtransferase | ||
| Rv0848 (cysM3) | 20.1 | Probable cysteine synthase A | Yes | |
| Rv0851c | 2.9 | Probable short-chain dehydrogenase | ||
| Rv0988 | −6.5 | −3.2 | Predicted secreted hydrolaseb | |
| Rv1471 (trxB) | 3.2 | Probable thioredoxin | Yes | |
| Rv1908c (katG) | 5.3 | Catalase peroxidase | Yes | |
| Rv2199c | −7.7 | −3.1 | Probable cytochrome c oxidase polypeptide 4b | |
| Rv2641 (cadI) | 4.1 | 71.6 | Predicted glyoxylase I enzymeb | Yes |
| Rv2962c | 5.0 | Probable glycosyl transferase | ||
| Rv3117 (cysA3) | 3.3 | Probable thiosulfate sulfur transferase | ||
| Chaperone | ||||
| Rv0350 (dnaK) | 3.0 | Molecular chaperone | ||
| Hypothetical | ||||
| Rv0057 | −4.9 | −3.9 | Hypothetical protein | |
| Rv0140 | 7.3 | Hypothetical protein | Yes | |
| Rv0190 | 8.5 | Hypothetical protein | Yes | |
| Rv0430 | −3.3 | Hypothetical protein | Yes | |
| Rv0500B | −4.6 | −3.1 | Hypothetical protein | |
| Rv0968 | 1.6 | 7.7 | Hypothetical protein | |
| Rv2466c | 5.5 | Hypothetical protein | Yes | |
| Rv3463 | 1.9 | 3.2 | Hypothetical protein | Yes |
a
Genes were identified as genes that were previously associated with oxidative stress based on identification in the hydrogen peroxide stress study of Schnappinger et al. (24).
b
Annotation based on BLASTP searches (http://www.ncbi.nlm.nih.gov/BLAST/).