TABLE 5.
Rate of l-rhamnose phosphorylationa
| Strain | Relevant genotype | Activity (μmol/min/mg) |
|---|---|---|
| Rlt100 | Wild type | 426 |
| Rlt100/pMR110 | Wild type rhaK+ | 300 |
| Rlt146 | rhaK52 | 12 |
| Rlt243 | ΔrhaU | 382 |
| Rlt243/pMR110 | ΔrhaU rhaK+ | 461 |
| Rlt243/pW3C1 | ΔrhaU rha+ | 479 |
a
Strains were grown in minimal medium (VMM) supplemented with 15 mM l-rhamnose-glycerol; induced cell extract was isolated and processed as described in Materials and Methods. Values are presented as one representative set of data. Data are the average of two independent replicates.